Nom i cognoms / Name and surname
Afiliació / Affiliation
IRB – BSC
Programa de finançament europeu en que s’enmarca aquest projecte? / European funding programme in which this project is being carried out?
Marie Skłodowska-Curie Schemes
Títol del projecte / Project title
Número del projecte / Project number
Breu explicació del projecte / Brief explanation of your project
NORAD is a conserved and highly expressed lncRNA that regulates genomic stability, and it has been implicated in cancer and other disease processes.
Previous characterizations of NORAD were only based on sequence-based computational inferences and have identified a modular organization of NORAD composed of several repeat units (NRUs).
Here, we experimentally determined for the first time the secondary structure of NORAD using in-vitro enzymatic probing coupled to Illumina sequencing (nextPARS approach) to provide a thorough characterization of NORAD structure.
Our results suggest that the spacer regions provide structural stability to NRUs. Additionally, we uncover two previously unreported NRUs and determine the core structural motifs conserved across NRUs.
Given the current interest in understanding the structure, function and evolution of long, non-coding RNAs and the clinical relevance of NORAD, we think that eventually, this may open the door to design novel therapeutic strategies in the fight against cancer.
Enllaç a la pàgina web del projecte / Link to your project website
Repte en que s’emmarca aquest projecte / Challenge within the framework of this project
1. Health, demographic change and wellbeing